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plko 1 puro constructs  (Addgene inc)


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    Structured Review

    Addgene inc plko 1 puro constructs
    Plko 1 Puro Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+puro/pLKO%2E1+puro+(Plasmid+%238453)/pmc13010945-62-9-16
    Average 96 stars, based on 1549 article reviews
    plko 1 puro constructs - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    shRNA:

    Article Title: BEX2 influences the MCL1-Hedgehog signaling axis to regulate the potential of stemness characterization in colorectal cancer.
    Article Snippet: Establishment of stable BEX2 overexpression cell lines BEX2 overexpression in HCT116 cells was achieved using the lentiviral-based vector, pLent-RFP-Blasticidin-CMV (Addgene, Invitrogen, Carlsbad, CA). .. The lentiviral-based vector, pLKO.1-Puro (Addgene), was utilized for shRNA expression. .. BEX2 was amplified and cloned into the pCMV-HA vector (Addgene, Invitrogen, Carlsbad, CA) using a BEX2 DNA fragment as a template in the transfection of HEK 293 cells.

    Article Title: ALKBH3 m1A Demethylase Deficiency Reduces Alzheimer's Amyloid-β Pathology.
    Article Snippet: All plasmids were transfected to cell lines with Lipofectamine 3000 (#L300015, Thermo Fisher, USA) according to the manufacturer’s instructions. .. Lentiviral short-hairpin RNA (shRNA) constructs for human ALKBH3 were obtained according to the pLKO.1-puro (#8453, Addgene, USA) vector protocol. ..

    Expressing:

    Article Title: BEX2 influences the MCL1-Hedgehog signaling axis to regulate the potential of stemness characterization in colorectal cancer.
    Article Snippet: Establishment of stable BEX2 overexpression cell lines BEX2 overexpression in HCT116 cells was achieved using the lentiviral-based vector, pLent-RFP-Blasticidin-CMV (Addgene, Invitrogen, Carlsbad, CA). .. The lentiviral-based vector, pLKO.1-Puro (Addgene), was utilized for shRNA expression. .. BEX2 was amplified and cloned into the pCMV-HA vector (Addgene, Invitrogen, Carlsbad, CA) using a BEX2 DNA fragment as a template in the transfection of HEK 293 cells.

    Knockdown:

    Article Title: AURKA-mediated destabilization of SAPS3 drives ferroptosis evasion via 7-dehydrocholesterol biosynthesis in colorectal cancer
    Article Snippet: For plasmid transfection, experiments were conducted with LipofectamineTM 3000 Transfection Reagent (Invitrogen, L3000008) according to the manufacturer’s instructions. .. To establish stable AURKA knockdown cell lines, shRNAs targeting the genomic sequences of AURKA were inserted into pLKO.1-puro (RRID: Addgene_8453) empty vector. .. Lentiviral particles were produced by transfecting HEK293T cells with pMD2.G (RRID: Addgene_12259), psPAX2 (RRID: Addgene_12260), and pLKO.1-shRNA plasmids at a 1:2:2 ratio using Lipo8000 TM reagent (Beyotime, C0533) for 48 h. Parental cells were then infected with shRNA-containing lentivirus for 24 h and selected with puromycin for 1–2 weeks.

    Article Title: AURKA promotes radioresistance in pancreatic cancer via GSK3β-mediated PTEN phosphorylation at T366.
    Article Snippet: .. For GSK3β knockdown, three shRNAs targeting GSK3β were designed using the TRC algorithm, and the most effective sequence was cloned into pLKO.1-puro (Addgene; Cat# 8453). ..

    Article Title: AURKA-mediated destabilization of SAPS3 drives ferroptosis evasion via 7-dehydrocholesterol biosynthesis in colorectal cancer.
    Article Snippet: For plasmid transfection, experiments were conducted with LipofectamineTM 3000 Transfection Reagent (Invitrogen, L3000008) according to the manufacturer's instructions. .. To establish stable AURKA knockdown cell lines, shRNAs targeting the genomic sequences of AURKA were inserted into pLKO.1-puro (RRID: Addgene_8453) empty vector. .. Lentiviral particles were produced by transfecting HEK293T cells with pMD2.G (RRID: Addgene_12259), psPAX2 (RRID: Addgene_12260), and pLKO.1-shRNA plasmids at a 1:2:2 ratio using Lipo8000TM reagent (Beyotime, C0533) for 48h.

    Genomic Sequencing:

    Article Title: AURKA-mediated destabilization of SAPS3 drives ferroptosis evasion via 7-dehydrocholesterol biosynthesis in colorectal cancer
    Article Snippet: For plasmid transfection, experiments were conducted with LipofectamineTM 3000 Transfection Reagent (Invitrogen, L3000008) according to the manufacturer’s instructions. .. To establish stable AURKA knockdown cell lines, shRNAs targeting the genomic sequences of AURKA were inserted into pLKO.1-puro (RRID: Addgene_8453) empty vector. .. Lentiviral particles were produced by transfecting HEK293T cells with pMD2.G (RRID: Addgene_12259), psPAX2 (RRID: Addgene_12260), and pLKO.1-shRNA plasmids at a 1:2:2 ratio using Lipo8000 TM reagent (Beyotime, C0533) for 48 h. Parental cells were then infected with shRNA-containing lentivirus for 24 h and selected with puromycin for 1–2 weeks.

    Article Title: AURKA-mediated destabilization of SAPS3 drives ferroptosis evasion via 7-dehydrocholesterol biosynthesis in colorectal cancer.
    Article Snippet: For plasmid transfection, experiments were conducted with LipofectamineTM 3000 Transfection Reagent (Invitrogen, L3000008) according to the manufacturer's instructions. .. To establish stable AURKA knockdown cell lines, shRNAs targeting the genomic sequences of AURKA were inserted into pLKO.1-puro (RRID: Addgene_8453) empty vector. .. Lentiviral particles were produced by transfecting HEK293T cells with pMD2.G (RRID: Addgene_12259), psPAX2 (RRID: Addgene_12260), and pLKO.1-shRNA plasmids at a 1:2:2 ratio using Lipo8000TM reagent (Beyotime, C0533) for 48h.

    Clone Assay:

    Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer
    Article Snippet: .. These gene blocks were then cloned into the pLKO.1-Puro (Addgene, #8453) plasmid and then transduced as described above into luciferase labeled MDA-MB-231 cells. ..

    Article Title: Targeting PI3Kδ suppresses pancreatic cancer by dual disruption of fibrosis and immune evasion.
    Article Snippet: .. The pMSCVhygro-Cre (Addgene plasmid # 34565) was a gift from Kai Ge.61 The tet-pLKO-puro was a gift from Prof. Dmitri Wiederschain (Addgene plasmid #21915).60 The pLKO.1 puro (Addgene plasmid #8453), pCMV-VSV-G (Addgene plasmid #8454) and pCMV-dR8.2 dvpr (Addgene plasmid #8455) were a gift from Prof. Bob Weinberg.59 The shRNAs were obtained from Sigma Aldrich and cloned into the Tet-pLKO-puro, pLKO.1 puro or pLKO.1 Hygro plasmid after digestion with AgeI/EcoRI. ..

    Article Title: AURKA promotes radioresistance in pancreatic cancer via GSK3β-mediated PTEN phosphorylation at T366.
    Article Snippet: .. For GSK3β knockdown, three shRNAs targeting GSK3β were designed using the TRC algorithm, and the most effective sequence was cloned into pLKO.1-puro (Addgene; Cat# 8453). ..

    Plasmid Preparation:

    Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer
    Article Snippet: .. These gene blocks were then cloned into the pLKO.1-Puro (Addgene, #8453) plasmid and then transduced as described above into luciferase labeled MDA-MB-231 cells. ..

    Article Title: Targeting PI3Kδ suppresses pancreatic cancer by dual disruption of fibrosis and immune evasion.
    Article Snippet: .. The pMSCVhygro-Cre (Addgene plasmid # 34565) was a gift from Kai Ge.61 The tet-pLKO-puro was a gift from Prof. Dmitri Wiederschain (Addgene plasmid #21915).60 The pLKO.1 puro (Addgene plasmid #8453), pCMV-VSV-G (Addgene plasmid #8454) and pCMV-dR8.2 dvpr (Addgene plasmid #8455) were a gift from Prof. Bob Weinberg.59 The shRNAs were obtained from Sigma Aldrich and cloned into the Tet-pLKO-puro, pLKO.1 puro or pLKO.1 Hygro plasmid after digestion with AgeI/EcoRI. ..

    Luciferase:

    Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer
    Article Snippet: .. These gene blocks were then cloned into the pLKO.1-Puro (Addgene, #8453) plasmid and then transduced as described above into luciferase labeled MDA-MB-231 cells. ..

    Labeling:

    Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer
    Article Snippet: .. These gene blocks were then cloned into the pLKO.1-Puro (Addgene, #8453) plasmid and then transduced as described above into luciferase labeled MDA-MB-231 cells. ..

    Multiple Displacement Amplification:

    Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer
    Article Snippet: .. These gene blocks were then cloned into the pLKO.1-Puro (Addgene, #8453) plasmid and then transduced as described above into luciferase labeled MDA-MB-231 cells. ..

    Construct:

    Article Title: ALKBH3 m1A Demethylase Deficiency Reduces Alzheimer's Amyloid-β Pathology.
    Article Snippet: All plasmids were transfected to cell lines with Lipofectamine 3000 (#L300015, Thermo Fisher, USA) according to the manufacturer’s instructions. .. Lentiviral short-hairpin RNA (shRNA) constructs for human ALKBH3 were obtained according to the pLKO.1-puro (#8453, Addgene, USA) vector protocol. ..

    Sequencing:

    Article Title: AURKA promotes radioresistance in pancreatic cancer via GSK3β-mediated PTEN phosphorylation at T366.
    Article Snippet: .. For GSK3β knockdown, three shRNAs targeting GSK3β were designed using the TRC algorithm, and the most effective sequence was cloned into pLKO.1-puro (Addgene; Cat# 8453). ..



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