plko 1 puro constructs (Addgene inc)
96
Structured Review
Addgene inc
plko 1 puro constructs
Plko 1 Puro Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro/pLKO%2E1+puro+(Plasmid+%238453)/pmc13010945-62-9-16
Average 96 stars, based on 1549 article reviews
Plko 1 Puro Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+puro/pLKO%2E1+puro+(Plasmid+%238453)/pmc13010945-62-9-16
Average 96 stars, based on 1549 article reviews
plko 1 puro constructs - by Bioz Stars,
2026-09
96/100 stars
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shRNA:Article Title: BEX2 influences the MCL1-Hedgehog signaling axis to regulate the potential of stemness characterization in colorectal cancer. Article Snippet: Establishment of stable BEX2 overexpression cell lines BEX2 overexpression in HCT116 cells was achieved using the lentiviral-based vector, pLent-RFP-Blasticidin-CMV (Addgene, Invitrogen, Carlsbad, CA). .. The lentiviral-based vector, Article Title: ALKBH3 m1A Demethylase Deficiency Reduces Alzheimer's Amyloid-β Pathology. Article Snippet: All plasmids were transfected to cell lines with Lipofectamine 3000 (#L300015, Thermo Fisher, USA) according to the manufacturer’s instructions. .. Lentiviral short-hairpin RNA (shRNA) constructs for human ALKBH3 were obtained according to the Expressing:Article Title: BEX2 influences the MCL1-Hedgehog signaling axis to regulate the potential of stemness characterization in colorectal cancer. Article Snippet: Establishment of stable BEX2 overexpression cell lines BEX2 overexpression in HCT116 cells was achieved using the lentiviral-based vector, pLent-RFP-Blasticidin-CMV (Addgene, Invitrogen, Carlsbad, CA). .. The lentiviral-based vector, Knockdown:Article Title: AURKA-mediated destabilization of SAPS3 drives ferroptosis evasion via 7-dehydrocholesterol biosynthesis in colorectal cancer Article Snippet: For plasmid transfection, experiments were conducted with LipofectamineTM 3000 Transfection Reagent (Invitrogen, L3000008) according to the manufacturer’s instructions. .. To establish stable AURKA knockdown cell lines, shRNAs targeting the genomic sequences of AURKA were inserted into Article Title: AURKA promotes radioresistance in pancreatic cancer via GSK3β-mediated PTEN phosphorylation at T366. Article Snippet: .. For GSK3β knockdown, three shRNAs targeting GSK3β were designed using the TRC algorithm, and the most effective sequence was cloned into Article Title: AURKA-mediated destabilization of SAPS3 drives ferroptosis evasion via 7-dehydrocholesterol biosynthesis in colorectal cancer. Article Snippet: For plasmid transfection, experiments were conducted with LipofectamineTM 3000 Transfection Reagent (Invitrogen, L3000008) according to the manufacturer's instructions. .. To establish stable AURKA knockdown cell lines, shRNAs targeting the genomic sequences of AURKA were inserted into Genomic Sequencing:Article Title: AURKA-mediated destabilization of SAPS3 drives ferroptosis evasion via 7-dehydrocholesterol biosynthesis in colorectal cancer Article Snippet: For plasmid transfection, experiments were conducted with LipofectamineTM 3000 Transfection Reagent (Invitrogen, L3000008) according to the manufacturer’s instructions. .. To establish stable AURKA knockdown cell lines, shRNAs targeting the genomic sequences of AURKA were inserted into Article Title: AURKA-mediated destabilization of SAPS3 drives ferroptosis evasion via 7-dehydrocholesterol biosynthesis in colorectal cancer. Article Snippet: For plasmid transfection, experiments were conducted with LipofectamineTM 3000 Transfection Reagent (Invitrogen, L3000008) according to the manufacturer's instructions. .. To establish stable AURKA knockdown cell lines, shRNAs targeting the genomic sequences of AURKA were inserted into Clone Assay:Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer Article Snippet: .. These gene blocks were then cloned into the Article Title: Targeting PI3Kδ suppresses pancreatic cancer by dual disruption of fibrosis and immune evasion. Article Snippet: .. The pMSCVhygro-Cre (Addgene plasmid # 34565) was a gift from Kai Ge.61 The tet-pLKO-puro was a gift from Prof. Dmitri Wiederschain (Addgene plasmid #21915).60 The Article Title: AURKA promotes radioresistance in pancreatic cancer via GSK3β-mediated PTEN phosphorylation at T366. Article Snippet: .. For GSK3β knockdown, three shRNAs targeting GSK3β were designed using the TRC algorithm, and the most effective sequence was cloned into Plasmid Preparation:Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer Article Snippet: .. These gene blocks were then cloned into the Article Title: Targeting PI3Kδ suppresses pancreatic cancer by dual disruption of fibrosis and immune evasion. Article Snippet: .. The pMSCVhygro-Cre (Addgene plasmid # 34565) was a gift from Kai Ge.61 The tet-pLKO-puro was a gift from Prof. Dmitri Wiederschain (Addgene plasmid #21915).60 The Luciferase:Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer Article Snippet: .. These gene blocks were then cloned into the Labeling:Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer Article Snippet: .. These gene blocks were then cloned into the Multiple Displacement Amplification:Article Title: Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancer Article Snippet: .. These gene blocks were then cloned into the Construct:Article Title: ALKBH3 m1A Demethylase Deficiency Reduces Alzheimer's Amyloid-β Pathology. Article Snippet: All plasmids were transfected to cell lines with Lipofectamine 3000 (#L300015, Thermo Fisher, USA) according to the manufacturer’s instructions. .. Lentiviral short-hairpin RNA (shRNA) constructs for human ALKBH3 were obtained according to the Sequencing:Article Title: AURKA promotes radioresistance in pancreatic cancer via GSK3β-mediated PTEN phosphorylation at T366. Article Snippet: .. For GSK3β knockdown, three shRNAs targeting GSK3β were designed using the TRC algorithm, and the most effective sequence was cloned into |